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KMID : 0379119910190020143
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Korean Journal of Mycology
1991 Volume.19 No. 2 p.143 ~ p.147
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Production of Pectolytic Enzymes by Botryosphaeria dothidea
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Abstract
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Botryosphaeria dothidea causing apple fruit rot was cultured in pectin-polypectate mineral salts or apple mediumss, to investigate pectolytic enzyme production and activity. Exo-polygalacturonase(PG) and exo-polymethylgalacturonase (PMG) in apple medium showed maximum of activity to 6.4 and 7.2 units at six days of culture, respectively. Their maximum activity in pectin-polypectate mineral salts medium was 5.9 and 5.3 units at eight days of culture lower than in apple medium respectively. Endo-PG and endo-PMG in pecin-polypectate mineral salts medium were maximum of activity to 4.4 and 16.2 units at six and eight days of culture, respectively, but activities in apple medium were 3.2 and 6.7 units at eight days of culture. Activity of polygalacturonate-trans-eliminase(PGTE) and pectinmethyl-traps-eliminase(PMTE) was higher in pectin-polypectate mineral salts medium than in apple medium. Fungal growth was maximum at six and eight days of culture in pectin-polypectate mineral salts and apple medium, respectively.
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